磷酸二酯酶I 返回列表頁(yè)

英文名稱(chēng)：Phosphodiesterase I from Crotalus adamanteus venom；5′-Exonuclease；Oligonucleate 5′-nucleotidohydrolase

其他名稱(chēng)：磷酸二脂酶；磷酸二酯酶

級(jí)別：BR

Specific activity：≥20u/mg protein

活力定義：One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25℃, pH 8.9.

產(chǎn)品描述：Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is compley inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg²⁺

性狀(以下信息僅供參考)：白色或類(lèi)白色粉末

用途：本品僅供科研，不得用于其它用途。(以下用途僅供參考)用于序列核酸的研究

保存：-20℃