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背景介紹:The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene
別名:26S protease regulatory subunit 6B; 26S proteasome AAA ATPase subunit RPT3; 26S proteasome AAA-ATPase subunit RPT3; MB67 interacting protein; MB67-interacting protein; MIP224; Protease 26S subunit 6; Proteasome (prosome macropain) 26S subunit ATPase 4; Proteasome 19S S6; Proteasome 26S subunit ATPase 4; Proteasome 26S subunit, ATPase, 4; PRS6B_HUMAN; PSMC4; RPT3; S6; Tat binding protein 7; TAT-binding protein 7; TBP 7; TBP-7.
Tat結合蛋白7抗體,Anti-TBP7抗體簡單介紹:
產品編號:BYK-18836R
英文名稱:Anti-TBP7
中文名稱:Tat結合蛋白7抗體
產品規格:50ul;100ul;200ul;1ml,*
抗體來源 Rabbit or Mouse
克隆類型 Polyclonal or Monoclonal
交叉反應、產品應用 (具體交叉反應與產品應用請或添加客服直接索取)
性 狀 Lyophilized or Liquid
濃 度 1mg/ml
Tat結合蛋白7抗體,Anti-TBP7抗體產品信息:
博研生物小課堂:
常用激光器對應熒光素的選擇:
藍色激光器熒光素(488nm):Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7
紅色激光器熒光素(633nm):APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647
黃色激光器熒光素(561nm):PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594
流式抗體選擇標準
確定流式細胞儀的參數配置
在設計流式方案前,先確定需要使用流式細胞儀檢測的型號,對該儀器的參數配置要了解以下幾個方面信息:
激光器:常見的流式細胞儀有488nm和635nm/633nm兩個激光器,但有些機器在購買時因某些原因,只裝了一個488nm激光器,所以需要注意型號相同的機器也未必激光配置相同。
濾光片:也就是有幾個檢測通道,這決定了你zui多可以同時檢測幾個指標。
儀器的具體型號:有助于再次確定檢測通道。因為同一個熒光在不同的流式細胞儀上檢測,有時候檢測通道也是不一樣的。
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