Rad51樣蛋白1抗體,Anti-Rad51L1抗體 返回列表頁

背景介紹：The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. Rearrangements between this locus and high mobility group AT-hook 2 (HMGA2, GeneID 8091) have been observed in uterine leiomyomata

別名：DNA repair protein RAD51 homolog 2; hREC2; MGC34245; OTTHUMP00000212251; OTTHUMP00000212253; OTTHUMP00000212254; OTTHUMP00000212255; R51H2; RA51B_HUMAN; RAD51 homolog B (S. cerevisiae); RAD51 homolog B; RAD51 like 1; RAD51 like protein 1; RAD51-like protein 1; Rad51B; RAD51L1; REC2; RecA like protein; Recombination repair protein.  

Rad51樣蛋白1抗體,Anti-Rad51L1抗體簡單介紹：

產品編號：BYK-19727R

英文名稱：Anti-Rad51L1
中文名稱：Rad51樣蛋白1抗體

產品規格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應、產品應用 （具體交叉反應與產品應用請或添加客服直接索取）

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

Rad51樣蛋白1抗體,Anti-Rad51L1抗體產品信息：

實驗注意事項

1、 把聚丙烯酰胺凝膠中的蛋白質電泳轉移到硝酸纖維膜上；

a. 轉移緩沖液洗滌凝膠和硝酸纖維素膜，將硝酸纖維素膜鋪在凝膠上，用5ml移液管在凝膠上來回滾動去除所有的氣泡

b. 在凝膠/濾膜外再包一張3mm濾紙（預先用轉移緩沖液浸濕），將凝膠夾在中間，保持濕潤和沒有氣泡

c. 將此濾紙/凝膠/薄膜濾紙按照廠家建議方法放入電泳裝置中，凝膠面向陰極

d. 將上述裝置放入緩沖液槽中，并灌滿轉移緩沖液以淹沒凝膠

e. 按照廠家所示接通電源開始電泳轉移

f. 轉移結束后，取出薄膜和凝膠，棄去凝膠

2、 將薄膜漂在氨基黑中快速染色，直至分子量標準顯現時取出，記錄下標準位置；

3、 用100ml水洗滌纖維素膜，必要時可用脫色緩沖液；

4、 膜置印跡緩沖液中于37℃保溫1小時；

5、 室溫下，用PBS-Tween緩沖液洗滌薄膜

6、 用封口機將薄膜封入塑料袋中，盡可能不留空氣；

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