淋巴細(xì)胞抗原6H抗體，Anti-LY6H抗體 返回列表頁

淋巴細(xì)胞抗原6H抗體，Anti-LY6H抗體簡單介紹：

產(chǎn)品編號：BYK-18455R

英文名稱：Anti-LY6H
中文名稱：淋巴細(xì)胞抗原6H抗體

別名：Ly-6H; LY 6H; Lymphocyte antigen 6 complex locus H; Lymphocyte antigen 6H; NMLY 6; NMLY6.  

背景介紹：The Ly-6 (Lymphocyte antigen 6) alloantigens represent a family of phosphatidylinositol-anchored proteins that play a role in the process of T lymphocyte activation. Ly-6 family members share amino acid homology throughout a distinctive cysteine rich protein domain that incorporates O-linked carbohydrates. During hematopoiesis, murine Ly-6 molecules have unique patterns of tissue expression, from multipotential stem cells to lineage committed precursor cells, and on specific leukocyte subpopulations in the peripheral lymphoid tissues. Ly-6H (Lymphocyte antigen 6H), also known as NMLY6, is a 140 amino acid lipid-anchored membrane protein that belongs to the Ly-6 family. Highly expressed in brain with lower expression in pancreas, testis, colon and small intestine, Ly-6H participates in the activation of T lymphocytes. Overexpression of Ly-6H is detected in certain acute human leukemic cell lines (such as MOLT-3), suggesting a possible role for Ly-6H in carcinogenesis.

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

交叉反應(yīng)、產(chǎn)品應(yīng)用 （具體交叉反應(yīng)與產(chǎn)品應(yīng)用請或添加客服直接索取）

性    狀 Lyophilized or Liquid
濃    度 1mg/ml

淋巴細(xì)胞抗原6H抗體，Anti-LY6H抗體產(chǎn)品信息：

合適的抗體稀釋度

抗體的濃度是免疫染色的關(guān)鍵，如果抗體濃度過高，抗體分子過多于抗原決定簇，可導(dǎo)致抗體結(jié)合減少，產(chǎn)生陰性結(jié)果。此陰性結(jié)果并不一定是缺少抗原，而是由于抗體過量，這種現(xiàn)象類似于凝集反應(yīng)中的前帶效應(yīng)（prozone effect）。因此，必須使用一系列稀釋做“棋盤式效價滴定”，檢測抗體的合適稀釋度，以得到zui大強度的特異性染色和zui弱的背景染色。抗體稀釋度應(yīng)根據(jù)：1.抗體效價高，溶液中特異性抗體濃度越高，工作稀釋度越高；2.一般講，應(yīng)用的抗體稀釋度越大，溫育時間越長；3.對于抗體與非特異性蛋白的結(jié)合，只有高稀釋度時才能防止其非特異性背景染色；4.稀釋用緩沖液的種類，標(biāo)本的固定和處理過程等也可影響稀釋度，所以合適的稀釋度應(yīng)根據(jù)具體情況測定。抗體的稀釋主要是指*抗體，因為*抗體中特異性抗體合適的濃度是關(guān)鍵，應(yīng)用高稀釋度*抗體僅高親和力的特異性染色反應(yīng)，減少或消除其中交叉抗體反應(yīng)。

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