• <del id="qqie6"><sup id="qqie6"></sup></del>
  • <tfoot id="qqie6"></tfoot>
  • <ul id="qqie6"></ul>
  • 上海勁馬實驗設備有限公司
    免費會員

    當前位置:首頁   >>   資料下載   >>   Plant VB12 FOR RESEARCH USE ONLY

    ELISA試劑盒
    進口ELISA試劑盒
    國產ELISA試劑盒
    生物試劑
    血清
    細胞
    標準品對照品
    進口培養基
    抗體
    食品檢測試劑盒
    放免試劑盒
    免疫化學產品
    其它方法測試盒

    Plant VB12 FOR RESEARCH USE ONLY

    時間:2013-7-16閱讀:551
    分享:
    • 提供商

      上海勁馬實驗設備有限公司
    • 資料大小

      112.5KB
    • 資料圖片

    • 下載次數

      99次
    • 資料類型

      WORD 文檔
    • 瀏覽次數

      551次
    點擊免費下載該資料

     

     
    Assay range:30ng/L - 850ng/L 96 determinations
    Purpose
    This kit allows for the determination of VB12 concentrations in Plant tissue ,cell and other
    samples.
    Principle of the assay
    The kit assay Plant VB12 level in the sample,use Purified Plant VB12 antibody to coat
    microtiter plate wells, make solid-phase antibody, then add VB12 to wells, Combined VB12
    antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after
    washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP
    enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the
    color change is measured spectrophotometrically at a wavelength of 450 nm. The
    concentration of Plant VB12 in the samples is then determined by comparing the O.D. of the
    samples to the standard curve.
    Materials provided with the kit
    1 wash solution 20ml× 1bottle 7 Stopp Solution 6ml× 1 bottle
    2 HRP-Conjugate reagent 6ml× 1 bottle 8
    Standard
    (1600ng/L)
    0.5ml× 1 bottle
    3 Microelisa stripplate 12well× 8strips 9 Standard diluent 1.5ml× 1bottle
    4 Sample diluent 6ml× 1 bottle 10 Instruction 1
    5 Chromogen Solution A 6ml× 1 bottle 11
    Closure plate
    membrane
    2
    6 Chromogen Solution B 6ml× 1 bottle 12 Sealed bags 1
    Specimen requirements
    RD
    2
    1. extract as soon as possible after Specimen collection,and according to the relevant
    literature, and should be experiment as soon as possible after the extraction. If it can’t,
    specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
    2. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
    Assay procedure
    1. Dilute and add sample:Dilute Original density Standard as follow table:
    800ng/L 5 Standard 150μl Original density Standard+150μl Standard diluent
    400ng/L 4 Standard 150μl 5 Standard+150μl Standard diluent
    200ng/L 3 Standard 150μl 4 Standard+150μl Standard diluent
    100ng/L 2 Standard 150μl 3 Standard +150μl Standard diluent
    50ng/L 1 Standard 150μl 2 Standard +150μl Standard diluent
    2.add sample:Set blank wells separay (blank comparison wells don’t add sample and
    HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample
    dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is
    5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
    3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
    4.Configurate liquid: 30-fold(or 20-fold) wash solution diluted 30-fold (or 20-fold) with distilled
    water and reserve.
    5.washing:Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer
    to every well, still for 30s then drain, repeat 5 times, dry by pat.
    6.add enzyme:Add HRP-Conjugate reagent 50μl to each well, except blank well.
    7.incubate:Operation with 3.
    8.washing:Operation with 5.
    9.color:Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the
    light preservation for 15 min at 37℃
    10.Stop the reaction:Add Stop Solution50μl to each well, Stop the reaction(the blue color
    change to yellow color).
    11.assay:take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and
    3
    within 15min.
    Steps description
    Standard, Sample diluent
    Add Standard, Sample diluent, incubate for 30 min at 37℃.
    Wash 5 time,Add HRP-Conjugate reagent, incubate for 30 min at 37℃.
    Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at 37℃.
    Add Stopp Solution
    Read absorbance at 450nm within 15 min
    calculate
    Calculate
    Take the standard density as the horizontal, the OD value for the vertical ,draw the
    standard curve on graph paper, Find out the corresponding density according to the sample
    OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line
    4
    regression equation of the standard curve with the standard density and the OD value ,with the
    sample OD value in the equation, calculate the sample density, multiplied by the dilution factor,
    the result is the sample actual density.
    Important notes
    1. The kit takes out from the refrigeration environment should be balanced 15-30 minutes in
    the room temperature, ELISA plates coated if has not use up after opened, the plate should
    be stored in Sealed bag.
    2. washing buffer will Crystallization separation, it can be heated the water helps dissolve
    when dilute . Washing does not affect the result.
    3. add Sample with sampler Each step, And proofread its accuracy frequently, avoids the
    experimental error. add sample within 5 min, if the number of sample is much , recommend
    to use Volley .
    4. if the testing material content is excessively higher (The sample OD is bigger than the first
    standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution
    factor.(× n× 5).
    5. Closure plate membrane only limits the disposable use, to avoid cross-contamination.
    6. The substrate evade the light preservation.
    7. Please according to use instruction strictly, The test result determination must take the
    microtiter plate reader as a standard.
    8. All samples, washing buffer and each kind of reject should according to infective material
    process.
    9. Do not mix reagents with those from other lots.
    Storage and validity
    1.Storage: 2-8℃.
    2.validity: six months

    會員登錄

    ×

    請輸入賬號

    請輸入密碼

    =

    請輸驗證碼

    收藏該商鋪

    X
    該信息已收藏!
    標簽:
    保存成功

    (空格分隔,最多3個,單個標簽最多10個字符)

    常用:

    提示

    X
    您的留言已提交成功!我們將在第一時間回復您~
    在線留言
    主站蜘蛛池模板: 国产成人亚洲综合无码| 欧美成人精品福利在线视频| 国产成人aaa在线视频免费观看| 成人伊人青草久久综合网破解版| 成人免费无遮挡无码黄漫视频| 国产成人精品久久一区二区小说 | 亚洲国产成人精品女人久久久 | 国产成人高清亚洲一区久久| 国产成人午夜福利在线观看视频| 亚洲国产一成人久久精品| 成人欧美在线视频| 亚洲国产成人精品无码区在线观看| 亚洲最大成人网色| 国产精品成人无码久久久| 中文国产成人精品久久一| 国产成人亚洲综合色影视| 欧美成人性色xxxxx视频大| 国产成人污污网站在线观看| 成人美女黄网站色大色下载| 免费国产成人高清在线观看麻豆| 成人污视频网站| 久久亚洲国产成人精品无码区| 国产成人综合在线视频| 成人妇女免费播放久久久| 亚洲国产成人九九综合| 天天影院成人免费观看| 成人片黄网站a毛片免费| 黑人粗长大战亚洲女2021国产精品成人免费视频 | 国产成人精品无码播放 | 亚洲欧洲精品成人久久曰影片| 成人午夜亚洲精品无码网站| 久久国产成人精品国产成人亚洲| 国产成人综合亚洲| 成人免费黄网站| 成人在线不卡视频| 成人免费毛片视频| 成人免费视频在线播放| 成人妇女免费播放久久久| 成人污视频网站| 成人午夜福利视频镇东影视| 成人AAA片一区国产精品|