BLO-11

RC Starcher
J Madaras

1

frozen

See Propagation

adherent

Mus musculus （mouse）

fibroblast

Tissue: skeletal muscle
Cell type: fibroblast
Disease: lysyl oxidase deficiency

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negative

H-2b

30 days

male

The line was derived from a mouse carrying the blotchy mutation.
The cells are deficient in lysyl oxidase.
ATCC CCL-197 is an age, sex and strain matched control for this line.
Tested and found negative for ectromelia virus （mousepox）.

ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; fetal bovine serum, 10%

Remove medium, add fresh 0.1% trypsin for about 10 minutes, and centrifuge to remove trypsin. Resuspend cell pellet in fresh medium and dispense into new flasks. Subculture every 7 days.
Subc*tion ratio: A subc*tion ratio of 1:2 to 1:5 is recommended

Medium renewal: Twice per week
 

26043: Starcher BC , et al. Lysyl oxidase deficiency in lung and fibroblasts from mice with hereditary emphysema. Biochem. Biophys. Res. Commun. 78: 706-712, 1977. PubMed: 20889
26044: Starcher B , et al. Abnormal cellular copper metabolism in the blotchy mouse. J. Nutr. 108: 1229-1233, 1978. PubMed: 27591