ibidi細(xì)胞定位格子培養(yǎng)皿µ-Dish 35mm Grid-500
35mm具500um網(wǎng)格刻度 ibidi標(biāo)準(zhǔn)底部的培養(yǎng)皿
應(yīng)用：
1.細(xì)胞或細(xì)胞簇的再定位
2.細(xì)胞計(jì)數(shù)，例如：細(xì)胞轉(zhuǎn)染效率計(jì)算
3.細(xì)胞運(yùn)動(dòng)觀察
4.細(xì)胞顯微操作中單細(xì)胞處理
貨號(hào)：
| 貨號(hào) | 產(chǎn)品名稱(chēng) | 規(guī)格（個(gè)/盒） |
| 81148 | µ-Dish 35 mm,高壁，玻璃底，50微米格子培養(yǎng)皿 | 30 |
| 81168 | µ-Dish 35 mm,高壁，玻璃底，500微米格子培養(yǎng)皿 | 30 |
| 80156 | µ-Dish 35 mm,低壁，ibiTreat底部處理，500微米格子培養(yǎng)皿 | 60 |
| 80151 | µ-Dish 35 mm,低壁，無(wú)包被，500微米格子培養(yǎng)皿 | 60 |
| 81166 | µ-Dish 35 mm,高壁，ibiTreat底部處理，500微米格子培養(yǎng)皿 | 60 |
| 81161 | µ-Dish 35 mm,高壁，無(wú)包被，500微米格子培養(yǎng)皿 | 60 |
產(chǎn)品特點(diǎn)：
1.特別適合細(xì)胞或細(xì)胞簇的定位和重找回；
2.的ibidi標(biāo)準(zhǔn)底部設(shè)計(jì)，180um的蓋玻片尺寸底部厚度，適合高分辨率顯微觀察；
3.的 ibiTreat 底部包被，適合大多數(shù)細(xì)胞的貼壁培養(yǎng)；
4.特殊蓋部設(shè)計(jì)減小液體揮發(fā)。
技術(shù)特征：
1.直徑為35mm標(biāo)準(zhǔn)樣式的培養(yǎng)皿；
2.具500µm 網(wǎng)格刻度；
3.字母或數(shù)字標(biāo)記(A-U; 1-20)；
4.替代選擇的玻璃底部產(chǎn)品：µ-Slide 35 mm, high glass bottom with Grid-50 or with Grid-500；
5.的 ibiTreat 組織培養(yǎng)處理表面適合大多數(shù)細(xì)胞的貼壁生長(zhǎng)；
6.生物相容性材料制作，不含膠水，無(wú)細(xì)胞毒性影響；
7.特殊的蓋部設(shè)計(jì)，減小液體揮發(fā)。
產(chǎn)品規(guī)格：
| Grid repeat distance | 500 µm | Growth area | 3.5 cm2 |
| ? µ-Dish | 35 mm | Growth area | 21 mm |
| Volume high / low | 2 / 0.8 ml | Height with lid high / low | 14 / 9 mm |
| 底部 | Ibidi標(biāo)準(zhǔn) | ||
Lid with Lock Position
The special lock feature in all ibidi µ-Dishes enables the user to minimize evaporation. The lock position provides excellent conditions for long term studies in non humidified environments. Gas exchange (carbon dioxide, oxygen) during cell culture is maintained thanks to the gas permeable plastic material of the dish.
TIP: Use the locking feature only if minimal evaporation is
required, i.e., outside incubators, non humidified microscopy stages, etc.
| Optical properties | ||
| Refractive index (nD 589 nm) | 1.52 | 1.52 |
| Abbe number | 56 | 55 |
| Thickness | #1.5 (180 µm) | #1.5 (170 µm) |
| Material | Microscopy plastic | D 263M Schott borosilicate glass |
| Autofluorescence | Low | Low |
| Transmission | Very high (even ultraviolet) | High (ultraviolet restrictions) |
| Birefringence (DIC) | Low (DIC compatible*) | Low (DIC compatible*) |
| Other aspects | ||
| Surface modifications | ibiTreat – tissue culture treated Uncoated – hydrophobic | Only pure glass |
| Protein coatings | Possible | Possible |
| Gas permeable | Yes | No |
| Material flexibility | High | Low |
| Breakable | No | Yes |
| Recommended for | All kinds of fluorescence microscopy | TIRF and single photon |
